Monitoring the Lactic Acid Bacterial Diversity during Shochu Fermentation by PCR-Denaturing Gradient Gel Electrophoresis(Environmental Biotechnology)

スポンサーリンク

概要

• 論文の詳細を見る

• The presence of lactic acid bacteria (LAB) during shochu fermentation was monitored by PCR-denaturing gradient gel electrophoresis (DGGE) and by bacteriological culturing. No LAB were detected from fermented mashes by PCR-DGGE using a universal bacterial PCR primer set. However, PCR-DGGE using a new primer specific for the 16S rDNA of Lactococcus, Streptococcus, Tetragenococcus, Enterococcus, and Vagococcus and two primers specific for the 16S rDNA of Lactobacillus, Pediococcus, Leuconostoc, and Weissella revealed that Enterococcus faecium, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus nagelii, Lactobacillus plantarum, Lactococcus lactis, Leuconostoc citreum, Leuconostoc mesenteroides, and Weissella cibaria inhabited in shochu mashes. It was also found that the LAB community composition during shochu fermentation changed after the main ingredient and water were added during the fermentation process. Therefore, we confirmed that PCR-DGGE using all three primers specific for groups of LAB together was well suited to the study of the LAB diversity in shochu mashes. The results of DGGE profiles were similar to the results of bacteriological culturing. In conclusion, LAB are present during shochu fermentation but not dominant.

• 社団法人日本生物工学会の論文
• 2005-03-25

著者

• Okada Sanae

  Nodai Culture Collection Center Department Of Brewing Tokyo University Of Agriculture

• ENDO AKIHITO

  Nodai Culture Collection Center, Department of Brewing, Tokyo University of Agriculture

• Endo Akihito

  Nodai Culture Collection Center Department Of Brewing Tokyo University Of Agriculture

関連論文

• 101 Identification of Lactobacillus plantarum and Lactobacillus pentosus by photobiotin labeling DNA-DNA hybridization
• Monitoring the Lactic Acid Bacterial Diversity during Shochu Fermentation by PCR-Denaturing Gradient Gel Electrophoresis(Environmental Biotechnology)

スポンサーリンク