Isolation of Aryl-Phosphate Ester-Degrading Bacterium from Leachate of a Sea-Based Waste Disposal Site(ENVIRONMENTAL BIOTECHNOLOGY)

概要

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An aryl-phosphate ester (APE)-degrading bacterium was isolated from the leachate of a sea-based waste disposal site. The isolated APE-degrading bacterial strain YS-57 grew well in a medium containing glucose and NaCl, and degraded two types of APE: tricresyl phosphate and triphenyl phosphate. The optimal temperature, pH, and NaCl concentration for the growth of strain YS-57 were 30℃, 7.0, and 1.0%, respectively. Strain YS-57 grew at an APE concentration of 25 mg/l without being inhibited. APEs were degraded by the supernatant of the medium in which strain YS-57 was cultured, suggesting that the APE-degrading enzyme was released into the extracellular space in the logarithmic growth phase. The 16S rDNA sequence of strain YS-57 showed 95.6% similarity to that of Roseobacter gallaeciensis and the morphological properties were also comparable. Consequently, strain YS-57 was closely related to the genus Roseobacter.
公益社団法人日本生物工学会の論文
2004-12-25