Constructing an Amylolytic Brewing Yeast Saccharomyces pastorianus Suitable for Accelerated Brewing(BREWING AND FOOD TECHNOLOGY)

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概要

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• An amylolytic brewing yeast Saccharomyces pastorianus, free of vector sequences and drug-resistance genes, was constructed by disrupting the α-acetolactate synthase gene and introducing the α-amylase gene as a selective marker. The resulting recombinant strain was able to utilize starch as the sole carbon source and its α-acetolactate synthase activity was lowered by 30%. Fermentation tests confirmed that the diacetyl concentration and the residual oligosaccharide were reduced by 70% and 25%, respectively, in fermented wort by the recombinant strain, while the brewing performance of the recombinant strain was retained.

• 公益社団法人日本生物工学会の論文
• 2004-12-25

著者

• Zhang Guangyi

  Bioscience And Bioengineering College Hebei Economics And Business University

• Liu Zengran

  Bioscience And Bioengineering College Hebei Economics And Business University

• LIU SHIGUI

  Life Science College, Sichuan University

• Liu Shigui

  Life Science College Sichuan University

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• Constructing an Amylolytic Brewing Yeast Saccharomyces pastorianus Suitable for Accelerated Brewing(BREWING AND FOOD TECHNOLOGY)

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