Use of Chemostat Data for Modelling Extracellular-Inulinase Production by Kluyveromyces marxianus in a High-Cell-Density Fed-Batch Process

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Production of extracellular inulinase by low-cell-density (2 kg dry weight・m^<-3>) sucrose-limited chemostat cultures of Kluyveromyces marxianus obeyed saturated kinetics at delution rates ranging from 0.02 to 0.5 h^<-1>. A non-structured Monod-type equation, describing the relation between specific growth rate and specific extracellular-inuliase production rate, was used to fit experimental data. This equation was subsequently incorporated in a model for the production of biomass and extracellular inulinase in a high-cell-density (>100 kg dry weight・m^<-3>)fed-batch vulture of K. marxianus grown on sucrose. The model adequately desvribed biomass production in the fed-batch culture. However, the production of extracellular inulinase in the fed-batch process was slightly higher than predicted by the model. This observation may be related to differences in growth conditions between in the chemostat and fed-batch cultures.
公益社団法人日本生物工学会の論文
1995-01-25