Regioselective Bioconversion of Colchicine and Thiocolchicine into Their Corresponding 3-Demethyl Derivatives

概要

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A variety of plant cell cultures and microbial soil isolates were screened for their ability to specifically demethylate colchicine at the C-3 position. Among all plant cell cultures tested, the newly established Colchicum veriegatum culture was the only one able to demethylate colchicine, however unspecifically, yielding a mixture of 3-demethylcolchicine and 2-demethylcolchine. In contrast, two bacterial strains were found among more than 500 isolates tested which expressed highly regio-specific demethylation activity exclusively at C-3 of colchicine. The biocomversion product of the microorganisms, 3-demethylcolchicine, was completely excreted into the medium. The specific C-3 bioconversion of colchicine as well as of thiocolchicine by one of these strains, Bacillus IND-B 375,was characterized in function of substrate concentration and incubation time.
公益社団法人日本生物工学会の論文
1995-01-25