Protoplast Formation, Regeneration and Fusion in Bacillus polymyxa

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概要

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• Two-hour lysozyme treatment(1mg/ml)of mid-logarithmic phase Bacillus polymyxa cells at 37℃ resulted in 99% protoplasting efficiency. Among three typical regeneration media, HCP 1.5 gave the highest regeneration frequency of 1.8×10^<-2>. Protoplast fusion between B. polymyxa B1103 Str^r His^- and B1104 Tyr^- produced 39.6% recombinants of the total regenerants, Str^r His^+ Tyr^- and Str^r His^- Tyr^- being the major recombinant phenotypes.

• 公益社団法人日本生物工学会の論文
• 1991-06-25

著者

• Santos Susana

  Microbiology Laboratory Institute Of Biological Sciences College Of Arts And Sciences University Of

• RAYMUNDO ASUNCION

  Microbiology Laboratory, Institute of Biological Sciences, College of Arts and Sciences, University

• CADIZ-PADRE VIRGINIA

  Microbiology Laboratory, Institute of Biological Sciences, College of Arts and Sciences, University

• Raymundo Asuncion

  Microbiology Laboratory Institute Of Biological Sciences College Of Arts And Sciences University Of

• Cadiz-padre Virginia

  Microbiology Laboratory Institute Of Biological Sciences College Of Arts And Sciences University Of

関連論文

• Protoplast Formation, Regeneration and Fusion in Bacillus polymyxa

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