Investigation of Carbon and Phosphorus Sources in Cultural Media of a Selected Strain of Alginate-producing Azotobacter vinelandii

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概要

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• From Azotobacter vinelandii strain AE-IV, six strains were separated by a routine method, and one strain, N-II-1-2,was selected which produced alginate in highest yield. The product was identified by ion exchange column, thin-layer and gas-liquid chromatographies and by IR spectrometry to be an O-acetylated alginate. The O-acetyl group was found almost exclusively in the alginate fragments rich in mannuronic acid residues obtained by the action of lyase from a mollusc, Dolabella auricula. Glucose, mannitol, maltose, fructose, sorbitol, sucrose, raffinose and glycerol were used as carbon source in investigating cell growth and alginate productivity. Fructose and sorbitol were excellent for the cell growth but very poor for the production of alginate, while the reverse was the case with glucose, mannitol and maltose. Sucrose gave good yields of both cell mass and alginate. The optimal concentration of phosphate for bacterial growth in Burk's medium was found to be around 5.0 mM.

• 1981-02-25

著者

• Nakajima Mutsuyasu

  Department Of Agricultural Chemistry College Of Agriculture And Veterinary Medicine Nihon University

• OKABE ERI

  Department of Fisheries

• MUROOKA HARUYOSHI

  Department of Agricultural Chemistry, College of Agriculture and Veterinary Medicine, Nihon Universi

• NISHIZAWA KAZUTOSI

  Department of Fisheries

• Murooka Haruyoshi

  Department Of Agricultural Chemistry College Of Agriculture And Veterinary Medicine Nihon University

関連論文

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• Trends of Arbekacin-resistant MRSA Strains in Japanese Hospitals (1979 to 2000)
• Investigation of Carbon and Phosphorus Sources in Cultural Media of a Selected Strain of Alginate-producing Azotobacter vinelandii

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