Formation of Cellulases and Degradation of Cellulose by Several Fungi

概要

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Five strains of fungi (Aspergillus niger, Lenzites trabea, Myrothecium verrucaria, Trichoderma koningii and Trichoderma lignorum) were tested for the production of cellulolytic enzymes on pure glucose and on cellulose media. The most active strains belonging to the genera of Trichoderma, Aspergillus and Myrothecium, also secreting high activities of β-glucosidase, were grown in a bioreactor under defined conditions. Depending on the strain this procedure resulted in a manifold increase in cellulolytic activities. The culture filtrates were concentrated and standardized with respect to β-glucosidase activity and used for the hydrolysis of cellulose powder. With Trichoderma-cellulase, 46% conversion of crystalline cellulose to glucose was achieved within 48 hr. The ratio of cellobiose to glucose found in the hydrolysate, the amount of high molecular carbohydrates as well as the degree of hydrolysis widely depended on the type of cellulase used.
公益社団法人日本生物工学会の論文
1978-08-25