Influence of External Mass Transfer Limitation on Apparent Kinetic Parameters of Penicillin G Acylase Immobilized on NonPorous Ultrafine Silica Particles

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概要

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• Immobilization of enzymes on nonporous supports provides a suitable model for investigating the effect of external mass transfer limitation on the reaction rate in the absence of internal diffusional resistance. In this study, deacylation of penicillin G was investigated using penicillin acylase immobilized on ultrafine silica particles. Kinetic studies were performed within the low-substrate-concentration region, where the external mass transfer limitation becomes significant. To predict the apparent kinetic parameters and the overall effectiveness factor, knowledge of the external mass transfer coefficient, k_L a, is necessary. Although various correlations exist for estimation of k_L a, in this study, an optimization scheme was utilized to obtain this coefficient. Using the optimum values of k_L a, the initial reaction rates were predicted and found to be in good agreement with the experimental data.

• 公益社団法人日本生物工学会の論文
• 2002-02-25

著者

• Kheirolomoom Azadeh

  Sharif University Of Technology Department Of Chemical Engineering

• KHORASHEH FARHAD

  Sharif University of Technology, Department of Chemical Engineering

• FAZELINIA HOSSEIN

  Sharif University of Technology, Department of Chemical Engineering

• Khorasheh Farhad

  Sharif University Of Technology Department Of Chemical Engineering

• Fazelinia Hossein

  Sharif University Of Technology Department Of Chemical Engineering

関連論文

• Influence of External Mass Transfer Limitation on Apparent Kinetic Parameters of Penicillin G Acylase Immobilized on NonPorous Ultrafine Silica Particles

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