Productivity Improvement in L-Sorbose Biosynthesis by Fedbatch Cultivation of Gluconobacter oxydans(BIOCHEMICAL ENGINEERING)

概要

論文の詳細を見る
The effect of increased (100, 200 and 300 gl^<-1>) initial sorbitol concentrations (S_0) was investigated in the sorbitol to sorbose bioconversion process. Batch cultivations with a S_0 of 100, 200 and 300 gl^<-1> were completed at 10, 14 and 24 h with a corresponding overall sorbose productivity of 10.1, 14.3 and 12.4 gl^<-1> h^<-1> respectively. The decrease in sorbose productivity at S_0=300 gl^<-1> was attributed to the inhibition by sorbitol of culture growth and product formation. In order to eliminate substrate inhibition, two identical fed-batch cultivations were performed in which a highly concentrated (500 gl^<-1>) sorbitol solution was added at a constant feed rate (0.2lh^<-1>) in the exponential phase of growth. The Fed-batch culture initiated with S_0= 225 gl^<-1> exhibited an enhanced accumulation of sorbose (336.2 gl^<-1>) but with a processing time of 24 h, and a productivity of 14.0 gl^<-1> h^<-1>. While, the fed-batch culture initiated with S_0=100 gl^<-1> accumulated 279.7 gl^<-1> of sorbose with an increase in productivity of 17.6 gl^<-1> h^<-1> in 16 h.
社団法人日本生物工学会の論文
2002-07-25