Complete cDNA Sequence of Chitin Deacetylase from Gongronella butleri and Its Phylogenetic Analysis Revealed Clusters Corresponding to Taxonomic Classification of Fungi
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概要
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A cDNA library containing a chitin deacetylase (CDA) gene from a zygomycete Gongronella butleri was constructed and the complete gene was sequenced. The complete gene contains an open reading frame of 1290 nucleotides which encodes a sequence of 430 amino acid residues. The gene sequence consists of nucleotides encoding a polysaccharide deacetylase domain located in the middle, covering 34% of the entire sequence. This domain shares high sequence similarities with CDAs of fungi and other deacetylases including the nodb-like protein from Sinorhizobium meliloti and Bacillus subtilis, xylanase D from Cellulomonas fimi, and acetylxylan esterase A from Streptomyces lividans. The deduced amino acid sequence shows homology with CDAs from Mucor rouxii (48% identity) and Phycomyces blakesleeanus (30% identity). Phylogenetic analysis reveals that CDAs are clustered in accordance with the taxonomic classification of the fungi.
- 社団法人日本生物工学会の論文
- 2002-04-25
著者
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Tan T
Department Of Biological Sciences National University Of Singapore
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MAW THEINGI
Department of Biological Sciences, National University of Singapore
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TAN TECK
Department of Biological Sciences, National University of Singapore
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KHOR EUGENE
Department of Chemistry, National University of Singapore
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WONGI SEK
Department of Biological Sciences, National University of Singapore
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Wongi Sek
Department Of Biological Sciences National University Of Singapore
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Khor E
Department Of Chemistry National University Of Singapore
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Maw Theingi
Department Of Biological Sciences National University Of Singapore
関連論文
- Complete cDNA Sequence of Chitin Deacetylase from Gongronella butleri and Its Phylogenetic Analysis Revealed Clusters Corresponding to Taxonomic Classification of Fungi
- シンガポ-ルにおけるマングロ-ブ菌類とそれらの発生に影響の考えられるいくつかの要因〔英文〕 (Post-5th ISME菌学シンポジウム--菌類と環境)