Conformation of Adsorbed Bovine Serum Albumin Governing Its Desorption Behavior at Alumina-Water Interfaces
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概要
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The mode of initial adsorption of bovine serum albumin(BSA) onto positively charged Al_2O_3 particles was studies as a function of surface coverage(θ). The adsorption isotherm of BSA exhibited saturation(θ=1) and the existence of an inflection point at θ of 0.82. The relative numbers of ionic groups on a BSA molecule interacting with the Al_2O_3 surface at various θ were monitored by measuring the relative adsorption density of H^+ and OH^-, ([Γ_<H+>-Γ_<OH->]), for BSA-adsorbed Al_2O_3 using potentiometric titration. The [Γ_<H+>-Γ_<OH->] curves for Al_2O_3, BSA, and BSA-adsorbed Al_2O_3 at various KNO_3 concentrations showed a common intersection point(cip) which was the pH giving the acid-base equivalence point, respectively. Compared with the cip's of Al_2O_3(5.6) and BSA(5.2), the cip's of BSA-adsorbed Al_2O_3 were situated at points corresponding to more alkaline pH values over the θ range of 0.13 to 1.0. These results suggested that negatively charged groups, mainly carboxyl groups, on the BSA molecule electrostatically interacted with the Al_2O_3 surface. The degree of mainly carboxyl groups, on the BSA molecule electrostatically interacted with the Al_2O_3 surface. The degree of shift in the cip increased gradually with increasing θ from 0.13 to 0.70, while it decreased markedly over the θ range of 0.82 to 1.0. The variation in the cip reflected the change in the total number of ion pairs formed between BSA molecules and Al_2O_3. The initial rates of BSA desorption during alkali cleaning were low and almost constant over the θ range of 0.13 to 0.70, but increased markedly at θ higher than 0.82. It is suggested that the conformational changes of BSA adsorbed on Al_2O_3, involving changes in the relative magnitude of electrostatic interaction forces, occur discretely at θ of approximately 0.8.
- 公益社団法人日本生物工学会の論文
- 2000-07-25
著者
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FUKUZAKI SATOSHI
Industrial Technology Center of Okayama Prefecture
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Fukuzaki Satoshi
Industrial Technol. Center Of Okayama Prefecture Okayama
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Urano Hiromi
Industrial Technol. Center Of Okayama Prefecture Okayama
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