On-Line Parameter Estimation of Industrial Fermentation Process with an Alkaliphilic Cellulase-Producing Bacterium by Exhaust Gas Analysis

概要

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A method for on-line estimation of the fermentation paramenters (substrate, product, and cells) of an alkaliphilic Bacillus sp.by utilizing data on the O_2 and Co_2 tensions in the exhaust gas and the pH of the fermentation broth was investigated. The estimation was based on the stoichimetric relations concerning the weight of CO_2 formed, O_2 consumed, product formed, and substrate consumed by the bacterium and on the ratio of the mass of the product to that of the cell biomass. During the fermentation, in which the pH range was 7.8-8.8, the parameters were successfully estimated by considering the reactions of CO_2 to produce HCO_3-and/or CO^<-2>_3 in the liquid-phase as well as the desorption of CO_2 to the aeration gas. It was verified that the proposed estimation method is appliable to industrial-scale fermentation using an alkaliphilic cellulase-producing Bacillus sp. The correlation coefficients between the estimated and observed fermentation parameters exceeded 0.990, indicating that the method is capable of evaluating fermentation parameters with an accuracy satisfactory for industial use.
社団法人日本生物工学会の論文
2000-03-25