Development of an Industrially Stable Process for _L-Threonine Fermentation by an _L-Methionine-Auxotrophic Mutant of Escherichia coli

概要

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Escherichia coli KY10935, an _L-methionine-auxotrophic mutant, has the ability to produce 100 g/l of _L-threonine under limited conditions of _<DL>-methionine, but occasionally exhibits overgrowth with a concomitant decrease in _L-threonine production. We found that the growth of the producer depended not only on the availability of _<DL>-methionine but also on that of iron, and that the dependence on iron caused abnormal fermentations in response to fluctuations in the iron concentration among lots of corn steep liquor, a cheap natural material used as the production medium. To overcome this problem, culture conditions were re-optimized with respect to the amounts of both _<DL>-methionine and iron added in the medium so that the growth was controlled irrespective of the lot of corn steep liquor, to establish a stable industrial process with high productivity.
公益社団法人日本生物工学会の論文
2000-01-25