Differential Organization and Transcription of the cat_2 Gene Cluster in Aniline-Assimilating Acinetobacter lwoffii K24

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CatABC genes encode proteins that are responsible for the first three steps of one branch of the β-ketoadipate pathway involved in the degradation of various aromatic compound by bacteria. Aniline-assimilating Acinetobacter lwoffii K24 is known to have the two-catABC gene clusters (cat_1 and cat_2) on the chromosome (Kim et al., J.Bacteriol., 179:5226-5231, 1997). The order of the cat_2 gene cluster is carB_2A_2C_2, which has not been found in other bacteria. In this report, we analyzed the transcriptional pattern of the cat_2 gene cluster and completely sequenced a 5.8 kbp fragment containing the compactly clustered catB_2A_2C_2 genes and four ORFs. Similar to the ORF_<R1> of the cat_1 gene cluster, an ORF highly homologous with the catR gene was found 102 bp upstream of the catB_2 gene and was designated as ORF_<R2>. Three ORFs, one putative reductase component (ORF_<X2>) and two putative LysR family regulatory proteins (ORF_<Y2> ORF_<Z2>) were located next to the catC_2 gene in the opposite direction of the cat_2 gene cluster. Two ORFs, ORF_<X2> and ORF_<Y2> were significantly homologous with tdnB and tdnR of the aniline oxygenase complex of Pseudomonas putida UCC22. RT-PCR analysis and Northern blotting revealed that the catB_2 gene is independently transcribed and that the catA_2C_2 genes are cotranscribed. A primer extension assay revealed that transcription of the catA_2C_2 gene starts in the C-terminal region of the catB_2 gene. These results suggest that the cat_2 gene cluster may be under a different gene adaptation from other cat gene clusters.
1999-09-25

Differential Organization and Transcription of the cat_2 Gene Cluster in Aniline-Assimilating Acinetobacter lwoffii K24

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