Construction of Recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 for Ferulic Acid Biotransformation to Vanillin

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概要

• 論文の詳細を見る

• Recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 capable of ferulic acid biotransformation to vanillin were constructed using homologous recombination and a PCR based cloning strategy, respectively. In the liquid culture of P.putida BO14, 26.81±2.30 μg vanillin ml^<-1> of culture filtrate was detected. In the case of recombinant E.coli QEFCA8, 19.37±1.95 μg vanillin ml^<-1> of culture filtrate was detected. Results indicate that the strains could be useful for the biotechnological production of vanillin, a very important flavoring substance.

• 公益社団法人日本生物工学会の論文
• 1999-07-25

著者

• Okeke Benedict

  Bacteriology Unit International Centre For Genetic Engineering And Biotechnology Area Science Park:(

• VENTURI VITTORIO

  Bacteriology Unit, International Centre for Genetic Engineering and Biotechnology, Area Science Park

• Venturi Vittorio

  Bacteriology Unit International Centre For Genetic Engineering And Biotechnology Area Science Park

関連論文

• Construction of Recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 for Ferulic Acid Biotransformation to Vanillin

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