Expression of a Carrot Invertase Gene in Tobacco Suspension Cells Cultivated in Batch and Continuous Culture Conditions

概要

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Plant cells(Nicotiana tabacum)were genetically modified to produce an heterologous protein, the acidic invertase from carrot, and invertase production from suspension tobacco cells was investigated. Suspension cultures were grown in shake flasks and stirred bioreactor. Total invertase activity was growth related. A 75d continuous culture in 10l bioreactor was performed. Our study demonstrates the high potential of plant cell cultures for long term production of heterologous protein.
公益社団法人日本生物工学会の論文
1999-03-25