Optimization of n Variable Biological Experimentws by Evolutionary Operation-Factorial Design Technique

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概要

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• Since the traditional approach to optimization of biological systems based on the single variable search technique is incapable of detecting the true optimum when a number of chemicals are used together as inducers, the Evolutionary Operation (EVOP)-factorial design technique was applied successfully to optimize the concentrations of vitamin (biotin), metal ion (CaCL_2) and plant hormone (NAA) for maximizing enzyme (protease) production by Rhizopus oryzae (RO IIT RB-13, NRRL-21498) in solid state fermentation. Addition of inducers to an optimal level (biotin-2.5 ppm, CaCl_2-20 ppm and NAA-12.5 ppm) resulted in a 〜1.9-fold increase in protease production.

• 公益社団法人日本生物工学会の論文
• 1999-02-25

著者

• Banerjee Rintu

  Microbial Biotechnology & Downstream Processing Laboratory Department Of Agricultural & Food

• Bhattacharyya Bimal

  Microbial Biotechnology & Downstream Processing Laboratory Department Of Agricultural & Food

• TUNGA RASHBEHARI

  Microbial Biotechnology & Downstream Processing Laboratory, Department of Agricultural & Food Engine

• Tunga Rashbehari

  Microbial Biotechnology & Downstream Processing Laboratory Department Of Agricultural & Food

関連論文

• Optimization of n Variable Biological Experimentws by Evolutionary Operation-Factorial Design Technique

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