Use of the Yeast Hansenula polymorpha(Pichia angusta) to Remove Contaminating Sugars from Ethyl β-D-Fructofuranoside Produced during Sucrose Ethanolysis Catalysed by Invertase
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概要
- 論文の詳細を見る
Alkyl glycosides are interesting intermediates for the production of biodegradable surfactants. Synthesis of ethyl β-D-fructofuranoside by invertase-catalysed ethanolysis of sucrose has been extensively reported in literature. However, this procedure yields mixtures of glucose, fructose, sucrose and ethyl β-D-fructofuranoside. Purification of ethyl β-D-fructofuranoside from such mixtures by chromatographic methods is laborious, difficult to scale up and requires organic solvents. The yeast Hansenula polymorpha grows rapidly on glucose, fructose and sucrose. Sucrose hydrolysis in this yeast is catalysed by an intracellular α-glucosidase('maltase');consequently, H.polymorpha should by unable to hydrolyse ethyl β-D-fructofuranoside. Indeed, aerobic cultivation of H.polymorpha on sugar mixtures obtained by invertase-catalysed ethanolysis of sucrose resulted in the complete removal of contaminating sugars, leaving ethyl β-D-fructofuranoside as the sole organic compound in culture supernatants. Pure ethyl β-D-fructofuranoside was recovered from the supernatants by mixed-bed ion exchange chromatography with an 86% yield.
- 公益社団法人日本生物工学会の論文
- 1999-01-25
著者
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Dijken Johannes
Kluyver Institute Of Biotechnology Delft University Of Technology
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Pronk Jack
Kluyver Institute Of Biotechnology Delft University Of Technology
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Hoek Pim
Kluyver Institute Of Biotechnology Delft University Of Technology
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Van Hoek
Kluyver Institute Of Biotechnology Delft University Of Technology
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HEIJDEN ANNA
Waterman Institute, Delft University of Technology
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KALITERNA JANKO
The Netherlands and Pliva Research Institute
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RANTWIJK FRED
Kluyver Institute of Biotechnology, Delft University of Technology
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Rantwijk Fred
Kluyver Institute Of Biotechnology Delft University Of Technology
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Heijden Anna
Waterman Institute Delft University Of Technology
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Van Der
Waterman Institute