Production of Acetic Acid from Methanol by Thermophilic Methanosarcina sp. : Acetate Production as an Index in Abnormal Methane Fermentation

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概要

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• Production of approximately 80-160 μmol of acetic acid was observed in 1%(w/v)methanol culture media(10 ml)of thermophilic Methanosarcina sp.from which essential nutrients such as salts of NH_4^+, PO_4^<3-> K^+, Ca^<2+> and Mg^<2+> were removed. Similar acetic acid production was found when methane gas formation was controlled to approximately 50% by means of inhibitors of methyl group incorporation into coenzyme M(CoM), such as 2-bromoethanesulfonic acid, 2-chloroethanesulfonic acid and 2-mercaptopropionic acid, with varying results for different doses. Among inhibitors of methane formation from methyl-CoM, only in the presence of chloroform some acetic acid production was observed. We studied the mechanism of acetic acid formation through tracing of marked methyl radicals in a culture medium containing 5 μmol of 2-bromoethanesulfonic acid, using ^13C methanol and nuclear magnetic resonance(NMR).

• 公益社団法人日本生物工学会の論文
• 1998-08-25

著者

• YAMAGUCHI Makoto

  Institute for Solkid State Physics, The University of Tokyo

• MINAMI KIYOSHI

  Institute of Technology, Shimizu Corporation

• Minami Kiyoshi

  Institute Of Technology Shimizu Corporation

• Yamaguchi Makoto

  Institute Of Technology Shimizu Corporation

• Yamaguchi Makoto

  Institute For Solid State Physics The University Of Tokyo

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• Production of Acetic Acid from Methanol by Thermophilic Methanosarcina sp. : Acetate Production as an Index in Abnormal Methane Fermentation

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