Antisense-Mediated Inhibition of Arginase (CAR1) Gene Expression in Saccharomyces cerevisiae

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概要

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• Inhibition of Saccharomyces cerevisiae arginase (CAR1) gene expression was investigated using the antisense RNA technique. CAR1 DNA fragments containing the yeast CAR1 gene sequences from the transcription initiation site (-49) or translation initiation site (+1) to the +501 region were amplified using PCR and inversely fused to the yeast CYC1 promoter on the yeast YIp5 plasmid. These recombinant plasmids were transformed into yeast cells to construct strains containing CYC1 promoter-antisense CAR1 DNA in their chromosomal DNA. When the CAR1 DNA region from -120 to +552 was amplified by PCR, the CYC1 promoter-antisense CAR1 DNA plasmid transformants produced the same size of PCR fragments as vector only transformants, suggesting the recombinant plasmids did not integrate into the CAR1 loci. The level of arginase production by the recombinant trasformants markedly decreased to about 15% of the enzyme activity produced by the vector only transformants.

• 公益社団法人日本生物工学会の論文
• 2001-11-25

著者

• Park Heui-dong

  Department Of Food Science And Technology Kyungpook National University

• Woo Im-sun

  Department Of Food Science And Technology Kyungpook National University

• SHIN MIN-CHOUL

  Department of Food Science and Technology, Kyungpook National University

• Shin Min-choul

  Department Of Food Science And Technology Kyungpook National University

関連論文

• Antisense-Mediated Inhibition of Arginase (CAR1) Gene Expression in Saccharomyces cerevisiae

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