Determination of 1, 5-Anhydro-D-Glucitol on the Basis of Its Inhibitory Effect on Trehalase Activity

概要

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1, 5-Anhydro-D-glucitol (1, 5-AG) was found to inhibit trehalase and trehalose phosphorylase activities competitively, because of its structural similarity with D-glucose. Trehalase from Nocardia sp., one of the most 1, 5-AG-sensitive enzymes, was used in the determination of 1, 5-AG concentration, which is a useful marker for the diagnosis of diabetes. A good linear relationship was observed between 1, 5-AG concentration in the range of 0.02 to 1.0 mM and the extent of trehalase inhibition by 1, 5-AG. The 1.5-AG concentration range could be determined by estimating enzymatically the amount of the reaction product, D-glucose, produced by the trehalase.
社団法人日本生物工学会の論文
2001-08-25