Expression of the Cytoplasmic Domain of NodC as an Active Form in Drosophila S2 Cells

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概要

• 論文の詳細を見る

• NodC, a membrane protein that catalyzes the synthesis of the chitin oligosaccharide chain, was successfully produced in a soluble form. The truncated nodC gene encoding only the cytoplasmic domain that deletes the hydrophobic N-terminus expressed both cytoplasmic and secreted proteins in Drosophila Schneider 2 cells. The expressed protein maintained the ability to synthesize chitin oligosaccharides, primarily (GlcNAc)_4, similar to the native membrane-bound NodC. This evidence suggests that only the large hydrophilic loop of NodC is efficient for enzymatic activity. Moreover, immobilizing the soluble NodC to a solid phase has no effect on the enzymatic activity. This, anchoring NodC is not necessary for its activity.

• 公益社団法人日本生物工学会の論文
• 2001-03-25

著者

• Shi Xiaohua

  National Food Research Institute

• Niimi Setsuko

  National Food Research Institute

• Machida S

  National Food Research Institute

• Machida Sachiko

  National Food Research Institute

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• Expression of the Cytoplasmic Domain of NodC as an Active Form in Drosophila S2 Cells

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