Multiplex Reverse Transcription Polymerase Chain Reaction for Simultaneous Detection of Viruses in Gentian

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概要

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• The multiplex reverse transcription polymerase chain reaction (RT-PCR) was used to detect Broad bean wilt virus 2, Cucumber mosaic virus and Clover yellow vein virus. Using specific primers, the method enabled detection of the viruses with a high degree of sensitivity and specificity and identification of the virus species according to the size of the PCR product amplified. Each of the three viruses was also detected within a mixture of the viruses by this method. Of 247 field plants of gentian subjected to the multiplex RT-PCR, 88% were found to be infected with one or more of the viruses.

• 日本植物病理学会の論文
• 2002-05-25

著者

• SUZUKI Kazumi

  Iwate Biotechnology Research Center

• Kuroda Tomohisa

  Iwate Biotechnology Research Center:japan Society For The Promotion Of Science Domestic Research Fel

• URUSHIBARA Shoji

  Iwate Agricultural Research Center

• TAKEDA Ikuko

  Iwate Biotechnology Research Center

• NAKATANI Fusaharu

  Iwate Agricultural Research Center

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