Specific Detection of Tomato Pathotype of Verticillium dahliae by PCR Assays

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概要

• 論文の詳細を見る

• Verticillium dahliae Klebahn is the causal agent of tomato wilt disease. Isolates of V. dahliae can be classified based on pathogenicity to tomato, but the pathotypes are indistinguishable in morphology. We designed PCR primers for specific detection of isolates pathogenic to tomato (tomato pathotype) from the sequences of a pathotype-specific gene, vdtl. With the primer pair Tg5/Tc3, a PCR product (approximately 3.2 kb) specific to tomato pathotype was amplified from the genomic DNA of isolates. Using the primer pair, a tomato pathotype isolate was specifically detected from hypocotyls of inoculated tomato and eggplant. On the other hand, no amplification was observed from non-tomato pathotype isolates of V. dahliae, some other wilt pathogens of tomato and a healthy host plant. Therefore, the primer pair can be useful for pathotype-specific detection of V. dahliae as well as for diagnosis of wilt disease of tomato plant.

• 日本植物病理学会の論文
• 2002-05-25

著者

• AMEMIYA Yoshimiki

  Faculty of Horticulture, Chiba University

• Shishido Masahiro

  Faculty Of Horticulture Chiba University

• USAMI Toshiyuki

  Faculty of Horticulture, Chiba University

• ABIKO Mafumi

  Graduate School of Life Science, Tohoku University

• Abiko Mafumi

  Graduate School Of Life Science Tohoku University

• Usami Toshiyuki

  Faculty Of Horticulture Chiba University

• Amemiya Y

  Faculty Of Horticulture Chiba University

• Amemiya Yoshimiki

  Faculty Of Horticulture Chiba University

• Usami Toshiyuki

  Graduate School Of Horticulture Chiba University

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