Characterization of Verticillium dahliae Isolates from Potato on Hokkaido by Random Amplified Polymorphic DNA (RAPD) and REP-PCR Analyses
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概要
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Verticillium dahliae isolates from potato on the island of Hokkaido (potato isolates) and those belonging to pathotypes A (eggplant pathotype), B (tomato pathotype) and C (sweet pepper pathotype) were divided into three distinct groups by RAPD and REP-PCR. The three DNA groups I, II, III consisted of pathotypes A and C, pathotype B and potato isolates, respectively. The potato isolates were assigned to pathotype A on the basis of pathogenicity. Another set of potato isolates was further collected from eight potato cropping regions on Hokkaido to further examine the relationships among them in detail. Only one of these isolates was identified as DNA group II, but all the others were classified as DNA group III. Isolates from daikon, eggplant, and melon on Hokkaido also belonged to DNA group III. These results suggest that V. dahliae isolates from Hokkaido are unique at the DNA level and different from other pathotype A isolates in Japan.
- 2001-02-25
著者
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Kageyama K
Gifu Univ. Gifu Jpn
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Kageyama Koji
Faculty Of Agriculture Gifu University
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KOMATSU Tsutomu
Hokkaido Ornamental Plants and Vegetables Research Center
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SUMINO Akio
Hokkaido Central Agricultural Experiment Station
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Komatsu T
Hokkaido Ornamental Plants And Vegetables Res. Center Takikawa Jpn
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