Serological and Molecular Characterization of a High Temperature-recovered Virus Belonging to Tospovirus Serogroup IV

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A serologically and cytologically distinct gloxinia tospovirus(HT-1)previously isolated from a gloxinia plant infected with Impatiens necrotic spot virus(INSV)when propagated in a high-temperature environment was characterized. Rabbit antisera produced for INSV and Tomato spotted wilt virus(TSWV)nucleocapsids(N)failed to react with HT-1 proteins in western blot analysis. The HT-1 antibodies reacted strongly with homologous antigen but failed to react with INSV and TSWV. However, the HT-1 antiserum reacted in ELISA with Watermelon silver mottle virus(WSMV)from Taiwan and in western blot analysis with the WSMV N protein. A reciprocal test showed that the antiserum prepared against the N protein of WSMV also reacted with the HT-1 N protein in both ELISA and western blot analysis. DNA probes derived from the N gene of HT-1 or WSMV hybridized to RNAs prepared from plants infected with either virus. Stronger signals were obtained with homologous than with heterologous reactions. Neither probe detected INSV or TSWV. The M and S RNAs of HT-1 were sequenced. The M RNA contains two open reading frames(ORF);one in the sense orientation encoding a nonstructural(NSm)protein of 308-amino-acids(aa)and the other in the ambisense orientation, a 1122-aa precursor of G1 and G2 glycoproteins. The S RNA also contains two ORFs;one in the sense orientation encoding a nonstructural(NSs)protein of 439 aa and the other in the ambisense orientation, an N protein of 277 aa. HT-1 is destantly related to INSV and TSWV as shown by low nucleotide(40-52%)and amino acid(28-48%)similarities in the four ORF sequences. The HT-1 virus shares high nucleotide(76-81%)and amino acid(85-92%)similarities with WSMV and peanut bud necrosis virus(PBNV). Based on the serological properties and sequence data, we propose that HT-1 is a distinct species of serogroup IV in the genus Tospovirus. This is the first time that a tospovirus similar to those found in the Far East and in Southeast Asia has been identified in the US.
日本植物病理学会の論文
2000-05-25

Serological and Molecular Characterization of a High Temperature-recovered Virus Belonging to Tospovirus Serogroup IV

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