Comparison of rDNA-ITS Sequences between Potato and Tobacco Strains in Rhizoctonia solani AG-3

スポンサーリンク

概要

• 論文の詳細を見る

• Genetic diversity among 51 isolates of Rhizoctonia solani AG-3, representing potato and tobacco populations, was inferred from the sequences of the internal transcribed spacer(ITS)and 5.8S ribosomal RNA(rRNA)gene.The 5.8S rDNA sequence was completely conserved not only in AG-3, but across all the AG isolates examined, whereas the rDNA-ITS sequence was found to be variable among the isolates.The nucleotide sequence similarity in the ITS 1 region was high(96-100%)for isolates within each of the two populations, but was 91-92% for isolates from different populations.The AG-3 isolates had 56 to 91% sequence similarities in the ITS 1 region with R.solani isolates of the other AGs.Phylogenetic analysis based on the ITS-5.8S rDNA sequence data indicated that the different populations in AG-3 are distantly related to each other.Genetic divergence between the two populations was also supported by the results of DNA-DNA hybridization studies.This study suggests that AG-3 consists of two genetically isolated groups corresponding to separate subgroups:AG-3 PT(potato type)and AG-3 TB(tobacco type).Specific primer sets for the detection of the two AG-3 subgroups were developed from the aligned rDNA-ITS sequences.

• 日本植物病理学会の論文
• 2000-02-25

著者

• Carling Donald

  Department Of Plant Pathology University Of Alaska Fairbanks

• Yokosawa Ryozo

  Department Of General Education Health Sciences University Of Hokkaido

• Kuninaga Shiro

  Department Of General Education Health Sciences University Of Hokkaido

• TAKEUCHI Toru

  Hokkaido Central Agricultural Experiment Station, Naganuma

• Takeuchi Toru

  Hokkaido Central Agricultural Experiment Station Naganuma

関連論文

• Comparison of microbial community structure between soils suppressive and conducive to Fusarium-wilt of radish
• Classification of Rhizoctonia spp. using rDNA-ITS sequence analysis supports the genetic basis of the classical anastomosis grouping
• The advancing identification and classification of Rhizoctonia spp. using molecular and biotechnological methods compared with the classical anastomosis grouping
• Comparison of rDNA-ITS Sequences between Potato and Tobacco Strains in Rhizoctonia solani AG-3
• Evaluation of the effects of five QTL regions on Fusarium head blight resistance and agronomic traits in spring wheat (Triticum aestivum L.)
• Fine mapping of foxglove aphid (Aulacorthum solani) resistance gene Raso1 in soybean and its effect on tolerance to Soybean dwarf virus transmitted by foxglove aphid
• Genetic relationships of soybean cyst nematode resistance originated in Gedenshirazu and PI84751 on Rhg1 and Rhg4 loci

スポンサーリンク