Production and Properties of a Lipopetide Biosurfactant from Bacillus subtilis C9

概要

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A lipopeptide biosurfactant, C9-BS produced by Bacillus (B.) subtilis C9,emulsified hydrocarbons, vegetable oil and crude oil. A high yield of C9-BS was obtained from a culture of B. subtilis C9 using a carbohydrate substrate, while a hydrocarbon substrate inhibited the production of the biosurfactant. The optimum medium for the production of C9-BS was as follows (g/l) : glucose, 40; NH_4HCO_3,13.5; K_2HPO_4,10.5; NaH_2PO_4,1.5;MgSO_4・7H_2O, 0.5; MnSO_4・4H_2O, 0.05; yeast extract, 0.5. The initial pH was 8.0 and the culture temperature was 30℃. The biosurfactnat C9-BS was obtained by collection of the foam that overflowed int he fermentorculture. The production of the biosurfactant by B. subtilis C9 was growth-associated Under O_2-limited conditions, C9-BS was produced in a 3-fold higher yield compared to that under O_2-sufficient conditions. C9-BS is souble in ethanol, acetone, methanol, butanol, chloroform, dichloromethane and alkaline water. The biosurfactant C9-BS lowered the surface tension of water to 28.5 dyne/cm, and the CMC (critical micelleconcentration) was 40 μM. C9-BS was stable from pH 5.0 to pH 9.5,in incubation at 100℃ for 1h, and at a salt concentration of 1,000 mM for NaCl and 10 mM for CaCl_2.
社団法人日本生物工学会の論文
1997-07-25