Scale-Up of Bacillus thuringiensis Fermentation Based on Oxygen Transfer

概要

論文の詳細を見る
A correlation between spore productivity and K_La, was found for Bacillus thuringiensis var. kurstaki. Since insecticidal crystal proteins synthesis depends on the highly oxygen-dependent sporulation process, the fermentation process was scale-up on the basis of the product K_La p, where p is the fermentor total pressure. The process was scale-up from a 14-l fermentor to a 1,100-l fermentor. Both reactors did not have the same geometrical design. Even though the specific growth rate decreased with the change of scale, the scale-up had a positive effect, since the total fermentation time was decreased while the biomass yiled and the sporulation efficiency were unchanged. Therefore K_La p is a practical tool for scaling up B. thuringiensis processes where product synthesis depends on sporulation.
公益社団法人日本生物工学会の論文
1997-06-25