Cloning and Sequencing of a Nitrile Hydratase Gene from Pseudonocardia thermophila JCM3095

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概要

• 論文の詳細を見る

• We report here the cloning and sequencing of Pseudonocardia thermophila JCM3095 genomic DNA clones that encode a nitrille hydratease (NHase). The NHase exhibits high optimum temperature and heat stabilities, and requires Co^<2+> for high activity. The deduced amino acid sequence of the enzyme consists of a (203aa, 612bp) and β(233aa, 699bp) subunits. P. thermophila is a moderate thermophile, and this is the first report of an NHase gene from a thermophile.

• 公益社団法人日本生物工学会の論文
• 1997-05-25

著者

• Oikawa Toshihiro

  (present Address) Omuta Laboratory Mitsui Toatsu Chemicals Inc.

• YAMAKI TOSHIFUMI

  Life Science Laboratory, Mitsui Toatsu Chemicals Inc.

• ITO KIYOSHI

  Life Science Laboratory, Mitsui Toatsu Chemicals Inc.

• NAKAMURA TAKESHI

  Life Science Laboratory, Mitsui Toatsu Chemicals Inc.

• Yamaki Toshifumi

  Life Science Laboratory Mitsui Toatsu Chemicals Inc.

• Ito Kiyoshi

  Life Science Laboratory Mitsui Toatsu Chemicals Inc.

• Nakamura Takeshi

  Life Science Laboratory Mitsui Toatsu Chemicals Inc.

関連論文

• Cloning and Sequencing of a Nitrile Hydratase Gene from Pseudonocardia thermophila JCM3095

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