Quantitative Analysis of Pulp-Derived Soluble Oligosaccharides by an Enzymatic-HPLC Method

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概要

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• A simple, rapid enzymatic-HPLC method was devised for the quantitative analysis of pulp-derived soluble oligosaccharides. Cello-, xylo-, and manno-oligomers were completely hydrolyzed by a crude enzyme mixture(Acucelase) of cellulases and hemicellulases from Aspergillus aculeatus no. F-50 for 1.0 h at 50℃. After the Acucelase treatment, the neutral sugars formed were separated and quantified by high-performance anionexchange chromatography with pulsed amperometric detection (HPAEC-PAD). This method was successfully applied to the quantitative analysis of oligomers formed by the enzymatic hydrolysis of pulp.

• 公益社団法人日本生物工学会の論文
• 1996-12-25

著者

• Sakamoto Reiichiro

  (present Address)oji Corn Starch Co. Ltd.

• KONNO MITSUGI

  (Present address)Akita Jujo Chemicals Co. Ltd.,

• KAMAYA YASUSHI

  Japan Pulp and Paper Research Institute Inc.

• Konno Mitsugi

  (present Address)akita Jujo Chemicals Co. Ltd.

関連論文

• Quantitative Analysis of Pulp-Derived Soluble Oligosaccharides by an Enzymatic-HPLC Method
• Role of Endoglucanase in Enzymatic Modification of Bleached Kraft Pulp

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