Propionate Degradation by Mesophilic Anaerobic Sludge : Degradation Pathways and Effects of Other Volatile Fatty Acids

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The degradation of volatile fatty acids by flocculant mesophilic (35±2℃) syntrophic sludge grown in an anaerobic hybrid reactor fed with a mixture of propionate, n-butyrate and ethanol (1 : 1 : 2 on COD basis) at a volumetric loading rate of 3.7 kg COD per m^3 per day was examined. The propionate degradation rate amounted to 1.1 mmol per g volatile suspended solids per day. The same propionate degradation rate was measured in the presence of 10 mM acetate, but it decreased by 30% when 10 mM n-butyrate was added. ^<13>-Nuclear magnetic resonance spectroscopy demonstrated interconversion of 1% to 10% of both the propionate and butyrate pool, during their simultaneous degradation. Propionate formation from [2-^<13>C]butyrate was not via a direct decarboxylation. ^<13>C-Propionate was converted via at least three different pathways. The first pathway was syntrophic conversion of propionate via the randomising pathway, evidenced by scrambling of [3-^<13>C]propionate into [2-^<13>C]propionate. Secondly, reductive carboxylation occurred, i.e. [3-^<13>C]propionate and [2,3-^<13>C]propionate were partly (2 to 10%) converted into [4-^<13>C]butyrate and [3,4-^<13>C]butyrate, respectively. Reductive carboxylation probably involved a transcarboxylase, as ^<13>C-bicarbonate was not incorporated in the carboxyl group of butyrate. Thirdly, propionate was converted into higher fatty acids : [2,3-^<13>C]propionate was converted into [4,5-^<13>C]valerate and 2-methyl[2,3-^<13>C]butyrate.
公益社団法人日本生物工学会の論文
1996-10-25

Propionate Degradation by Mesophilic Anaerobic Sludge : Degradation Pathways and Effects of Other Volatile Fatty Acids

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