Immobilization of Cuscuta reflexa Starch Phosphorylase : Production of Glucose-1-Phosphate Using Bioreactors
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概要
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Starch phosphorylase (EC 2.4.1.1) from young filaments of Cuscuta reflexa was immobilized on egg shell. The immobilized enzyme showed an alkaline shift in the optimum pH and an increase in optimum temperature compared with the soluble enzyme. The immobilized enzyme also displayed increased thermostability at 30℃, with a half-life of 18 h; at this temperature, the half-life of the soluble enzyme was 6 h. The immobilized enzyme could be reused 44 times at 30℃, with a decline of 10% in the enzyme activity. The increase in thermostability and reusability of the immobilized enzyme was useful for the production of glucose-1-phosphate, a cytostatic agent used in cardiopathy therapy. Two types of reactors, packed bed and fluidized bed, were developed and operated using egg shell-bound starch phosphorylase. The fluidized bed reactor operated at 30℃ and a flow rate of 5.5 ml/h showed better potential for the production of glucose-1-phosphate. The product was obtained with 97% purity after adsorption chromatography on norite and ion exchange chromatography on DEAE cellulose.
- 社団法人日本生物工学会の論文
- 1996-04-25
著者
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Kumar A
Devi Ahilya Univ. Indore Ind
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Kumar Anil
School Of Biotechnology Devi Ahilya University
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Nighojkar Anand
(present Address)maharaja Ranjit Singh College Of Professional Sciences
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SRIVASTAVA SADHANA
School of Biotechnology, Devi Ahilya University
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Srivastava Sadhana
School Of Biotechnology Devi Ahilya University
関連論文
- Immobilization of Cuscuta reflexa Starch Phosphorylase : Production of Glucose-1-Phosphate Using Bioreactors
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