Stability and Metabolic Versatility of a Propionate-Degrading Biofilm Operating in an Anaerobic Fluidised Bed Reactor

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Pulse perturbation experimets performed in the steady-state of an anaerobic fluidised bed reactor, which has been operated with propionate as sole substrate for two years, have shown considerable metabolic versatility of the biofilm population. After having been fed pulsed additions of propionate or propionate plus sulfate, ethanol, formate, L(+)-lactate (in the presence and absence of propionate), and a racemic mixture of lactate (in the presence of propionate), all substrates were degraded with the exception of lactate. The propionate turnover was 71.4-75.2 mmol propionate・l^<-1>・d^<-1> equivalent to a high biomass specific propionate degradatoin of 287-302 μmol・mg protein・l^<-1>・d^<-1> at low propionate concentrations during the steady of reator operarion and 85.4 mmol propionate. l^<-1>・df^<-1> equivalent to 343 μmol・mg protein^<-1>・d^<-1> at high propionate concentrations after pulsed addition of propionate. L(+)-Lactate and recemic lactate addition resulted in a reduction of the propionate turnover to 26.9 and 11.6 mmol propionate・l^<-1>・d^<-1>, respectively. The propionate turnover ws not affected by the addition of sulfate, neither stimulating nor inhibiting, but in the presence of sulfate the electron flow showed a partition into sulfate reduction and methanogenesis. 22% of electrons released by propionate oxidation served to reduce sulfate, while 78% of propionate was still syntrophically degrade with concomitant methane-production. Infection of 1 mM sodium molybdaet into the reactor caused an immediate and total inhibition. The biofilm population was stable and could survive a 5.5 months starvation period without significant loss of activity
公益社団法人日本生物工学会の論文
1995-10-25

Stability and Metabolic Versatility of a Propionate-Degrading Biofilm Operating in an Anaerobic Fluidised Bed Reactor

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