Asymmetric Bioreduction of a β-Ketoester to (R)-β-Hydroxyester by the Fungus Mortierella alpina MF 5534
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概要
- 論文の詳細を見る
Two hundred and sixty strains of microorganisms, (60 strains of yeasts, 60 strains of bacteria and 140 strains of fungi), were evaluated for their ability to stereoselectively bioreduce a β-ketoester {[2S-(2α, 4β)]-1-[(1,1-dimethylethoxy)carbonyl]-4-hydroxy-β-oxo-2-pyrrolidinepropanoic acid 1,1-demethylethyl ester} to the corresponding (R)-β-hydroxyester {[2S-(2α(S^*), 4β]-β, 4-dihydroxy-1-[(1,1-dimethylethoxy)carbonyl]-2pyrrolidinepropanoic acid 1,1-dimethylethyl ester}, a precursor to the β-methyl carbapenem antibiotic BO 2727. Among all the mecrobes evaluated, only one fungal strain, Mortierella alpina MF 5534 (ATCC 8979) was found to catalyze the desired reaction. The scaled-up bioconversion process in laboratory bioreactor (23-l scale) supported (R)-β-hydroxyester titers of 550 mg/l during a 250-h cultivation cycle and allowed the timely production of gram quantities of diastereomerically pure materials (diastereomeric excess >98).
- 公益社団法人日本生物工学会の論文
- 1995-08-25
著者
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Katz Lorraine
Merck Research Laboratoies
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CHARTRAIN MICHEL
Merck Research Laboratories
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ARMSTRONG JOSEPH
Merck Research Laboratories
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KELLER JENNIFER
Merck Research Laboratories
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MATHRE DAVID
Merck Research Laboratories
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GRESHAM RANDOLPH
Merck Research Laboratories
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- Asymmetric Bioreduction of a β-Ketoester to (R)-β-Hydroxyester by the Fungus Mortierella alpina MF 5534
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