High Level Expression of Protein L, an Immunoglobulin-Binding Protein, in Escherichia coli

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概要

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• A high level expression system for production of an immunogulobulin-binding protein, in Escherichia coli was studied. The protein, called protein L_<I-IV>, consists of four immunogloburin-binding domains of the native protein L. A simple fed-batch cultivation strategy was used to investigate the influence of different induction times on cell growth, viability, acetic acid formation and product formation. Induction allowing product formatioin for several hours, i.e., in this case in early exponential phase, was most favorable in terms of product yields. The highset specific yield obtained was 150 mg protein per gram cell dry weight (dw), corresponding to 360 mg per liter broth. The leakage of product into the media was less than 5%. Induction in early expotential phase lead to the highest amount of acetic acid, 1.47 g/g dw. Viability decreased significantly after induction.

• 公益社団法人日本生物工学会の論文
• 1995-07-25

著者

• Holst Olle

  Dept. Of Biotechnology Chemical Center Lund University

• Tocaj Anita

  Dept. Of Biotechnology Chemical Center Lund University

• SJOBRING ULF

  Dept. of Medical Microbiology, Lund Univeresity

• BJORCK LARS

  Dept. of Medical and Physilogical Chemistry, Lund University

• Bjorck Lars

  Dept. Of Medical And Physilogical Chemistry Lund University

• Sjobring Ulf

  Dept. Of Medical Microbiology Lund Univeresity

関連論文

• High Level Expression of Protein L, an Immunoglobulin-Binding Protein, in Escherichia coli

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