On-Line Monitoring of Product Concentration by Flow-ELISA in an Integrated Fermentation and Purification Process

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概要

• 論文の詳細を見る

• Process integration involving fermentation with cell recycling, affinity adsorption to remove the product, and on-line process monitoring of the product were investigated.α-Amylase was produced in continuous and batch fermentation of Bacillus amyloliquefaciens with complete cell rexycling, which was achieved using a membrane unit coupled to the fermentor. An affinity column of crosslinked starch was employed in the rexycling loop to adsorb the α-amylase before the cell-free broth was recycled to the fermentor. The concentration of α-amylase in the cell-free broth prior to and after the affinity separaton step was continuoulsy registered using aflow-injecton, immunochemical monitoring system. The different unit operations were integrated pair-wise, and eventually as a complete system. The complete feasiblity of monitoring a macromolecular product on-line during a cultivation/adsorption process was also demonstrated.

• 社団法人日本生物工学会の論文
• 1994-11-25

著者

• Nilsson Mats

  Department Of Genetic And Pathology Uppsala University Rudbeck Laboratory

• Mattiasson Bo

  Department Of Biotechnology Chemical Center Lund University

• Mattiasson Bo

  Department Of Biotechnology Center For Chemistry And Chemical Engineering Lund University

• HOLST OLLE

  Department of Biotechnology, Centre for Chemistry and Chemical Engineering, Lund University

• Holst O

  Lund Univ. Lund Swe

• Holst Olle

  Department Of Biotechnology Centre For Chemistry And Chemical Engineering Lund University

• JIJAYAKUMAR ARUMUGASWAMY

  Department of Biotechnolory, Chemical Center, Lund University

• SCHORNACK CHRISTINA

  Department of Biotechnology, Chemical Center, Lund Universtiy

• HAKANSON HAKAN

  Department of Biotectnology, Chemical Center, Lund University

• Hakanson Hakan

  Department Of Biotectnology Chemical Center Lund University

• Schornack Christina

  Department Of Biotechnology Chemical Center Lund Universtiy

• Jijayakumar Arumugaswamy

  Department Of Biotechnolory Chemical Center Lund University

• Nilsson Mats

  Department Of Biotechnology Chemical Center Lund University

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