Enhancement of Glucose Oxidase Fermentation by Addition of Hydrocarbons

スポンサーリンク

概要

• 論文の詳細を見る

• Addition of a hydrocarbon to fermentation medium has been recognized as a means of enhancing oxygen transfer. However, the mechanism involved has not been clarified. We investigated the glucose oxidase fermentation using Aspergillus niger in the presence of n-dodecane, n-hexadecane and soybean oil. The hydrocarbons had positive influences on the formation of both intra- and extracellular glucose oxidases. Moreover, the fermentation time needed to achieve the maximum enzyme yield was reduced. The increase in the yield of glucose oxidase was due to an increase in the efficiency of enzyme synthesis in the cells for cases of n-dodecane of n-hexadecane, while it was due to an increase in cell concentration for soybean oil. The increase in the level of extracellular glucose oxidase arose from increased enzyme secretion. However, it was interesting to find that the hydrocarbons delayed the cell growth as well as the production of glucose oxidase. This fact implies that the hydrocarbons alone do not improve the oxygen transfer. An explanation was made by considering the timing of feeding of the antifoaming agent KM-70 into the fermentation broth. The improved effects of the hydrocarbons on the fermentation are due to their interaction with the antifoaming agent. Accordingly, the timing of addition of the hydrocarbons to the culture is an important determinant for the fermentation. Apparently, the optimal timing is shortly after the start of feeding of the antifoaming agent.

• 社団法人日本生物工学会の論文
• 1994-10-25

著者

• Chen T‐l

  National Cheng Kung Univ. Tainan Twn

• LI TAI-HSING

  Department of Chemical Engineering, National Cheng Kung University

• CHEN TEH-LIANG

  Department of Chemical Engineering, National Cheng Kung University

• Li T‐h

  National Cheng Kung Univ. Tainan Twn

• Li Tai-hsing

  Department Of Chemical Engineering National Cheng Kung University

• Chen Teh-liang

  Department Of Chemical Engineering National Cheng Kung University

関連論文

• Enhancement of Glucose Oxidase Fermentation by Addition of Hydrocarbons
• Production of an Alkaline Lipase by Acinetobacter radioresistens
• A STUDY OF OXYGEN-VECTORS WITH A MODEL OF OXIDATION OF GLUCOSE IN THE PRESENCE OF GLUCOSE OXIDASE AND CATALASE
• Fast calculation of elution curves of a slurry adsorber with nonlinear kinetics.
• Simulation of nonlinear fixed-bed adsorbers by orthogonal collocation with the aid of fast Fourier transform.

スポンサーリンク