Indirect Estimation of Cell Mass and Substrate Concentration Using a Computer-Coupled Mass Spectrometer

概要

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On line estimation of cell mass and substrate concentration based on exhaust gas analysis was developed. The O_2,CO_2,H_2O, and N_2 contents at the inlet and outlet of fermentor, analyzed by a computer-coupled quadrupole mass spectrometer, were used to calculated the ozygen uptake rate and carbon dioxide evoltution rate, and these rates were further used to evaluate cell mass and substrate concentration in a recombinant Escherichia coli fermentation. Cell mass, glucose concentration, specific growth rate, and specific consumption rate of glucose were well estimated by this method; the oxygen uptake rate gave more accurate estimates fro these state variables than did the carbon dioxide evolution rate.
公益社団法人日本生物工学会の論文
1994-03-25