Selective Isolation of β-Amylase Derepressed Mutants of Bacillus acidopullulytics

スポンサーリンク

概要

• 論文の詳細を見る

• An experiment was conducted to elucidate the mechanism of the regulation of β-amylase synthesis in Bacillus acidopullulyticus NCIMB11608. It was found that the synthesis of β-amylase was induced by starch and repressed by glucose. β-Amylase derepressed mutants of B. acidopullulyticus NCIMB11608 2were isolated by nitrosogianidine mutation, using a starch medium containing 2-deoxyglucose as a repressor for the catabolite repression-resistant mutants. One promising mutant, designated as CR27-9-2,was selected which possessed five times the β-amylase activity of its parental strain. It was also found that the differential rate of enzyme synthesis in mutant CR27-9-2 was consrtant, regardless of the presence or absence of starch. Therefore, it was concluded that the synthesis of β-amylase in mutant CR27-9-2 was both constitutive and catabolite repression-resistant. In addition, CR27-9-2 was also catabolite repression-resistant for the synthesis of other amylolytic enzymes, such as α-amylase, pullulanse, and glycoamylase.

• 社団法人日本生物工学会の論文
• 1994-03-25

著者

• Jen Wang

  Graduate Institute Of Food Science And Technology National Taiwan University

• CHUI YU

  Graduate Institute of Food Science and Technology, National Taiwan University

• Chui Yu

  Graduate Institute Of Food Science And Technology National Taiwan University

関連論文

• Selective Isolation of β-Amylase Derepressed Mutants of Bacillus acidopullulytics

スポンサーリンク