Regeneration of Plantlets from Celery (Apium graveolens L.) Callus Using a Fermentor

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The efficiency of somatic embryogenesis of calluses induced from celery (Apium graveolens L.) (F1 variety, 1026-2) in an air-lift type fermentor was about half of that in flasks. The efficiency of somatic embryo maturation in an air-lift type fermentor was almost the same as that in flasks, and the efficiency in a stirred-tank type fermentor was about 70% of that in an air-lift type. We succeeded in producing 1,590,000 somatic embryos, and 296,000 plantlets after maturation by batch culture using a 30-l air-lift type fermentor.
公益社団法人日本生物工学会の論文
1994-02-25