Medium Optimization by a Fractional Factorial Design for Lipase Production by Rhizopus delemar

概要

論文の詳細を見る
The aim of the present work is to improve production of extracellular lipase by Rhizopus delemar using industrial grade carbon and nitrogen sources and performing statistical optimization of the production medium.Soya paste and dextrin were selected in the first phaes of this work as the best carbon and nitrogen sources and were used in the following experiments. Optimization of the medium composition was carried out with a fractional factorial design in two steps. Lipolytic activity at 48 h was 12-fold greater than with the initial medium.The results show that it is necessary to modify the carbon source-nitrogen source ratio, to reduce olive oil concentration and to add Tween 80 to the medium to achieve maximum production.
社団法人日本生物工学会の論文
1993-08-25