Cloning and Characterization of SKT5,a Saccharomyces cerevisiae Gene That Affects Protoplast Regeneration and Resistance to Killer Toxin of Kluyveromyces lactis
スポンサーリンク
概要
- 論文の詳細を見る
Saccharomyces cerevisiae strains carrying a mutation in the SKT5 gene are resistant to the killer toxin of Kluyveromyces lactis and are defective in protoplast regeneration. To understand the role of the SKT5 gene in toxin sensitivity and protoplast regeneration, we initiated a molecular study of this gene. A DNA fragment that complemented both the skt5 phenotypes was isolated from a yeast genomic library constructed in a single cope vector YCp50. Deletion mapping revealed that the entire complementing activity was carried on a 2.0 kilobase fragment. The DNA sequence of this fragment was determined and a long open reading frame encoding 533 amino acids with a calculated molecular weight of 59,165 daltons was found in the DNA sequence. In a homology search of the GenBank and EMBL data bases, this gene product showed no significant sequence similarity to any known protein coding sequence. Chromosomal mapping by pulsed field gradient gel electrophoresis and Southern hybridization analyses revealed that the SKT5 gene was a single copy gene located on chromosome II. The 2.0 kilobase transcipt transcript of this gene was detected by Northern analysis. A genomic disruption of the SKT5 gene was constructed by the insertion of a 2 kilobase LEU2 fragment. Disruption of this gene was not lethal; cells carrying the disrupted skt5 allele (skt5 : : LEU2) were viable and had phenotypes similar to those of cells carrying the original skt5 allele. Thus, the 59 kilodalton protein deduced from the DNA sequence analysis is indeed a product of the SKT5 gene and is unlikely to be essential for the growth of yeast cells.
- 社団法人日本生物工学会の論文
- 1992-10-25
著者
-
OHNO Takeshi
Laboratory for Planetary Sciences, Department of Earth and Planetary Sciences, Graduate School of Sc
-
Kawamoto Shinichi
Laboratory Of Molecular Biology Faculty Of Agriculture Hokkaido University
-
NOMURA MASAHIRO
Laboratory of Molecular Biology, Faculty of Agriculture, Hokkaido University
-
Ohno Takeshi
Laboratory Of Molecular Biology Faculty Of Agriculture Hokkaido University
-
Nomura Masahiro
Laboratory Of Molecular Biology Faculty Of Agriculture Hokkaido University
-
Nomura Masahiro
Laboratory Of Applied Entomology Graduate School Of Bioagricultural Science Nagoya University
関連論文
- Precise Zn Isotopic Ratio Measurements of Human Red Blood Cell and Hair Samples by Multiple Collector-ICP-Mass Spectrometry
- Cloning and Characterization of SKT5,a Saccharomyces cerevisiae Gene That Affects Protoplast Regeneration and Resistance to Killer Toxin of Kluyveromyces lactis
- Monitoring of organophosphate and carbamate resistance and identifying a combination of insecticides showing negatively correlated cross resistance in the green rice leafhopper (Nephotettix cincticeps Uhler) (Hemiptera : Deltocephalidae)
- Non-ant antiherbivore defenses before plant-ant colonization in Macaranga myrmecophytes
- Effects of synthesized tannin on the growth and survival of a generalist herbivorous insect, the common cutworm, Spodoptera litura Fabricius(Lepidoptera: Noctuidae)
- Variations in abiotic defense within myrmecophytic and non-myrmecophytic species of Macaranga in a Bornean dipterocarp forest