Factors Affecting Culture Fluorescence When Monitoring Bioreactors

概要

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Several key factors which affect culture fluorescence signals from bioreactor systems, especially, the fluorescence signals from excited tryptophan, pyridoxine, NADH and riboflavin, were studied. These factors included probe position, agitation and aeration rates, pH, temperature, dissolved oxygen, inner filter effects and cascade effects. Among these factors, inner filter effects, temperature, probe position, aeration and agitation rates were found to be most important in quantifying culture fluorescence signals. The pH effect was particularly important in quantifying tryptophan and pyridoxine fluorescence. Dissolved oxygen and cascade effects were not a serious problem for the four cellular fluorophores dissolved in buffer. Mathematical models were developed to calculate pH, temperature and inner filter effects on the back scattered fluorescence signals.
公益社団法人日本生物工学会の論文
1992-08-25