Partitioning and Separation of α-Lactalbumin and β-Lactoglobulin in PEG/Potassium Phosphate Aqueous Two-Phase Systems

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概要

• 論文の詳細を見る

• Bovine α-lactalbumin (α-La) and β-lactoglobulin (β-Lg) were partitioned in aqueous two-phase systems composed of polyethylene glycol (PEG) and potassium phosphate. The influences of several parameters on protein partition coefficients such as PEG molecular weight, phase component concentration, pH, and addition of NaCl were investigated and discussed in terms of possible mechanisms involved. From partition results, α-La and β-Lg in Cheddar cheese whey could separated by their differential partitionings in an aqueous two-phase system, where partially purified α-La and β-Lg could be recovered in the top and bottom phases, respectively. By using PEG with a low molecular weight (PEG 1000 or PEG 1500) and a high concentration of potassium phosphate (20% w/w), essentially pure β-Lg and 84% pure α-La from SDS-PAGE analysis could be obtained simultaneously from Cheddar chhese whey in a single liquid-liquid extraction step using aqueous two-phase systems.

• 公益社団法人日本生物工学会の論文
• 1992-02-25

著者

• Chen Jyh-ping

  Department Of Chemical Engineering Chang Gung College Of Medicine And Technology

• Chen Jyh-ping

  Department Of Chemical Engineering National Cheng Kung University

• CHEN JYH-PING

  Department of Chemical Engineering, National Cheng Kung University

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