Isolation of a Tetracyclinge Resistance Plasmid from a Glutamate-Producing Corynebacterium, Corynebacterium melassecola

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概要

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• A tetracycline resistance plasmid pAG1 (20.4 kb) and a cryptic plasmid pAG3 (4.5 kb) have been isolated from a non-pathogenic glutamate-producing corynebacteriu, Corynebacterium melassecola. A 3.1-kb BamHI/BglII fragment carrying the tetracycline resistance gene of pAG1 was isolated and ligated into the BamHI site of pAG3. A resultant small tetracycline resistance plasmid pAG50 (7.6 kb) was obtained. This tetracycline resistance marker is good enough to allow direct selection in transformation experiments.

• 社団法人日本生物工学会の論文
• 1990-09-25

著者

• Fujii M

  Japan Tobacco Inc. Shizuoka Jpn

• Takeda Y

  Toyama Univ. Toyama Jpn

• TAKEDA YASUHIKO

  (Current address)Foods Research Group, Asahi Chemical Industruy Co. Ltd.,

• FUJII MIKIO

  Asahi Chemical Industry Company Ltd.,

• NAKAJYOH YUKIHIRO

  Asahi Chemical Industry Company Ltd.,

• NISHIMURA TAKAAKI

  Asahi Chemical Industry Company Ltd.,

• ISSHIKI SADAO

  Asahi Chemical Industry Company Ltd.,

• Isshiki Sadao

  Asahi Chemical Industry Company Ltd.

• Nakajyoh Yukihiro

  Asahi Chemical Industry Company Ltd.

• Nishimura Takaaki

  Asahi Chemical Industry Company Ltd.

関連論文

• Cloning and Expression in Escherichia coli of the Glutamate Dehydrogenase Gene, gdh, from Corynebacterium melassecola
• Isolation of a Tetracyclinge Resistance Plasmid from a Glutamate-Producing Corynebacterium, Corynebacterium melassecola
• Comparison of the Second Alcohol Dehydrogenase Subunit Gene in Acetic Acid Bacteria
• Cloning of the pepX Gene of Lactobacillus helveticus IFO3809 Encoding Salt-Tolerant X-Prolyl Dipeptidyl Aminopeptidase and Characterization of the Enzyme

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