Biomethanation of Spent Wash : Heavy Metal Inhibition of Methanogenesis in Synthetic Medium

概要

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Five heavy metals detected in distillery waste were lead (1.0-8.8 μg/ml), copper (1.7-15.7 μg/ml), zinc (3.1-11.8 μg/ml), iron (36.0-43.5 μg/ml), and manganese (3.0-5.1 μg/ml). Their toxicity to biomethanogenesis in a synthetic medium containing 1% sodium acetate, propionate, or butyrate was measured by batch fermentation, after cultivating the bacterial biomass semicontinuously. Lead, copper, and zinc in decreasing order were found to be toxic to biomethanogenesis. Lead at the concentration of 10 μg/ml completely stopped methane production. Iron did not produce any notable change in the process while manganese stimulated the rate of methane production. The toxicity of lead, copper, and zinc to methanogenic bacteria and methane production was dose-dependent but the growth of acetogenic bacteria was impaired at higher concentrations (2.5-10.0 μg/ml) of lead, copper, and zinc. Manganese stimulated the growth of only methanogenic bacteria, but not that of non-methanogenic bacteria or acetic acid production. The reduction in the synthesis of acetic acid via butyrate was more in the presence of these three metals than the synthesis of this acid via propionate.
公益社団法人日本生物工学会の論文
1990-05-25