A New Dialysis Fermentor for the Production of High Concentrations of Extracellular Enzymes

概要

論文の詳細を見る
A new single-vessel dialysis fermentor is presented, which can be sterilized in situ and handled like the usual standard fermentors. The reactor is recommended for the production of high concentrations of extracellular enzymes. This is done in such a manner that the products are not spoiled through contact with the crude components of technical nutrient solutions. The reactor has been tested for the production of lipase with a culture of Staphylococcus carnosus. Sixty g・l^<-1> of cellular dry mass and 227 mg・l^<-1> lipase were produced within 46 h, using a continuously renewed high concentration of substrate. For planning the experiments and for the development of an optimal operation strategy, a mathematical model of the dialysis reactor system proved to be very helpful. The membrane permeability coefficient found for the limiting fraction of the yeast extract-peptone medium corresponds to a molecular weight between 300 and 400.
社団法人日本生物工学会の論文
1990-04-25