Continuous Conversion of Glutamine to Glutamate by Immobilized Glutaminase-Producing Yeast

概要

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Yeast cells with a salt-tolerant and thermostable glutaminase were immobilized in silica ges(S gel)and/or alginate-silica complex gel(AS gel). The inhibition rate of the conversion rate of immobilized cells by NaCl were lower that that of free cells. The glutaminases of immobilized cells and free cells were not inactivated by heat treatment at 60℃ for 1h. The half-lives of glutaminase in AS gel were 310d at 40℃, 40d at 45℃, and 14d at 50℃ at a constant space velocity(SV)of 0.64. The half-life of the glutaminase activity in cells immobilized in AS gel was longer than that in S gel. By passing a filtrate of wheat gluten hydrolyzed by proteolytic enzymes through the column containing the cells immobillized in AS gel at SV of 0.20,10mg/ml of glutamate was continuously produced.
公益社団法人日本生物工学会の論文
1990-03-25